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Nirogacestat dihydrobromide salt form · hydrobromide · Parent PF-3084014 · Synonyms: PF-3084014 dihydrobromide

Nirogacestat dihydrobromide is a reversible, orally bioavailable, noncompetitive, and selective γ-secretase inhibitor.

For research use only. Not for human or veterinary use.

Research area Cancer
Purity >98% Stock Inquire

Nirogacestat dihydrobromide structure

CAS No.: 1962925-29-6

Download structure (.mol)

Pack sizes & pricing

Size Price SKU Stock
100 mg USD 485.00 BP-01861A-100mg In stock Get quote
250 mg USD 785.00 BP-01861A-250mg Inquire Get quote
500 mg USD 1,185.00 BP-01861A-500mg Inquire Get quote
1 g USD 1,885.00 BP-01861A-1g In stock Get quote
2 g Inquire BP-01861A-2g Inquire Inquire

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Description

Nirogacestat dihydrobromide (PF-3084014 dihydrobromide) is a reversible, orally bioavailable, noncompetitive, and selective γ-secretase inhibitor with an IC50 of 6.2 nM. Inhibition of Notch signaling by Nirogacestat dihydrobromide while minimizing gastrointestinal toxicity presents a promising approach for research of Notch receptor-dependent cancers.

Biological activity

In Vitro The IC50 of Nirogacestat (PF-03084014) for γ-secretase enzyme inhibition in cell-free assay for Aβ production using detergent solubilized membranes derived from HeLa cells is determined to be 6.2 nM. When tested for inhibition of Notch receptor cleavage in cellular assays using HPB-ALL cells that harbor mutations in both the heterodimerization and PEST domains in Notch1, the cell IC50 is determined to be 13.3 nM. Nirogacestat causes a significant increase in caspase-3 activities in HPB-ALL and TALL-1 cells as well as an induction of cleaved PARP and cleaved caspase-3 after a 7-day treatment[1]. In Vivo Nirogacestat (PF-03084014) shows robust antitumor activity in this model on 14-day twice daily dosing. Tumor growth inhibition is dose dependent, with maximal tumor growth inhibition of ~92% obtained at high dose levels (150 mg/kg). In tumor growth inhibition studies where mice receive repetitive twice daily dosing for more than a week, Nirogacestat is well tolerated at dose levels below 100 mg/kg as no significant weight loss, morbidity, or mortality is observed. When the dose is increased to 150 mg/kg, however, mice have diarrhea and show weight loss (10-15%) approximately 10 days after compound administration. The body weight of treated animals usually returns to normal if dosing holidays are given, suggesting that the toxicity of Nirogacestat is reversible[1].

Identifiers

SMILES
FC1=C2C(CC[C@H](N[C@H](C(NC3=CN(C(CNCC(C)(C)C)(C)C)C=N3)=O)CCC)C2)=CC(F)=C1.Br
InChIKey
SPQUOBQFKZELBO-WCRWPNQISA-N

Specifications

MW (average)
651.4680
Formula
C27H43Br2F2N5O
CAS No.
1962925-29-6
Physical state
Solid
Color
White to yellow
Shipping
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage
Powder 4℃,sealed storage, away from moisture, 2 years; In solvent -20℃ 1 month;

Solubility

Soluble in DMSO

References

[1]. Wei P, et al. Evaluation of selective gamma-secretase inhibitor PF-03084014 for its antitumor efficacy and gastrointestinal safety to guide optimal clinical trial design. Mol Cancer Ther. 2010 Jun;9(6):1618-28.

Same target

Search γ-secretase

Same research area

Search Cancer

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