Aldehyde reactive probe free base · Synonyms: ARP

Aldehyde reactive probe (TFA) is a biotinylated reagent for the detection and quantification of AP sites in damaged DNA[1].

For research use only. Not for human or veterinary use.

Target Biochemical Assay Reagents Fluorescent Dye

Research area Others

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Data Sheet SDS COA

Aldehyde reactive probe structure

CAS No.: 627090-10-2

Download structure (.mol)

Pack sizes & pricing

Size	Price	SKU	Stock
100 mg	USD 498.00	BP-02396-100mg	In stock	Get quote
250 mg	USD 798.00	BP-02396-250mg	In stock	Get quote
500 mg	USD 1,398.00	BP-02396-500mg	In stock	Get quote
1 g	USD 2,198.00	BP-02396-1g	In stock	Get quote
2 g	Inquire	BP-02396-2g	In stock	Inquire

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Description

Aldehyde reactive probe (TFA) is a biotinylated reagent for the detection and quantification of AP sites in damaged DNA[1].

Biological activity

In Vitro Aldehyde reactive probe TFA (100 μL; 1 h at 37 °C) detects AP sites and related aldehyde-containing damages in DNA isolated from heat-inactivated, X-irradiated E. coli AB1157 cells, with signal intensity proportional to radiation dose[1]. Aldehyde reactive probe TFA (100 μL; 1 h at 37 °C) detects AP sites formed in the DNA of MMS-treated E. coli AB1157 cells. Its signal is proportional to the concentration of MMS and decreases during the repair process after treatment[1]. Aldehyde reactive probe TFA (2 mM; 1 h) specifically reacts with apurinic sites in acid-apurinized yeast tRNAPhe, and its reaction activity is directly proportional to the degree of apurinization

Identifiers

SMILES: C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NNC(=O)CON)NC(=O)N2.C(=O)(C(F)(F)F)O
InChIKey: YJYRTJQOEOYKFD-AEXHUXLESA-N

Specifications

MW (average): 445.4150
Formula: C₁₄H₂₂F₃N₅O₆S
CAS No.: 627090-10-2
Physical state: Solid
Color: White to off-white
Shipping: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage: Powder -20°C, sealed storage, away from moisture; In solvent -20°C, 1 month;

Solubility

soluble in DMSO

In vitro: DMSO : 25 mg/mL

Documents

References

[1]. Kubo K, et al. A novel, sensitive, and specific assay for abasic sites, the most commonly produced DNA lesion. Biochemistry. 1992;31(14):3703-3708.

[2]. Tanaka M, et al. An assay for RNA oxidation induced abasic sites using the Aldehyde Reactive Probe. Free Radic Res. 2011;45(2):237-247.

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