C12FDG free base · Synonyms: 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside

C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection.

For research use only. Not for human or veterinary use.

Target Fluorescent Dye

Research area Others

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Data Sheet SDS COA

C12FDG structure

CAS No.: 138777-25-0

Download structure (.mol)

Pack sizes & pricing

Size	Price	SKU	Stock
10 mg	USD 485.00	BP-01881-10mg	In stock	Get quote
25 mg	USD 785.00	BP-01881-25mg	In stock	Get quote
50 mg	USD 985.00	BP-01881-50mg	In stock	Get quote
100 mg	USD 1,350.00	BP-01881-100mg	In stock	Get quote
250 mg	USD 2,250.00	BP-01881-250mg	In stock	Get quote
500 mg	Inquire	BP-01881-500mg	Inquire	Inquire

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Description

C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di(β-D-galactopyranoside) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm) .

Biological activity

In Vitro Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solution preparation 1.1 Prepare storage solution Solvent: DMSO Concentration: 33 mM is recommended (e.g., weigh 1 mg C12FDG and dissolve it in 35.5 μL solvent). Storage: After aliquoting, store at -20°C or -80°C in the dark and avoid repeated freezing and thawing. 1.2 Preparation of working solution Dilute to 33 μM (optimized according to the experiment) with PBS (or other buffer). Note: The working solution should be prepared and used immediately, and protected from light. 2. Fluorescent senescence-associated β-galactosidase (SA-β-Gal) assay: 2.1 Culture cells in 6-, 12- , 24-, or 96-well plates at a density of 5× 105 cells/mL overnight. Incubate the cells according to your normal protocol. 2.2 Wash cells by 200 μL of PBS once and fix with 100 μL of fixation solution (2% formaldehyde/0.2% glutaraldehyde in distilled water) at room temperature for 5 min. 2.3 Wash cells by 200 μL of PBS two times, and stain with 100 μL of 33 μM C12FDG (in PBS, pH=6.0) for 10 min, and with 200 μL of Hoechst solution (1 μg/mL Hoechst 33342 (HY-15559) in PBS, pH 6.0) for 10 min. 2. 4 Image these cells by a 360-nm (Hoechst 33342) and 480-nm (C12FDG) excitation filters, and monitor through 460-nm and 535-nm emission filters, respectively.

Identifiers

SMILES: CCCCCCCCCCCC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O[C@H]6[C@@H]([C@H]([C@H]([C@@H](CO)O6)O)O)O)=C5)C=C(O[C@H]7[C@@H]([C@H]([C@H]([C@@H](CO)O7)O)O)O)C=C4)OC2=O)C=C1)=O
InChIKey: SLIWIQKBDGZFQR-PIVCGYGYSA-N

Specifications

MW (average): 853.9046
Formula: C₄₄H₅₅NO₁₆
CAS No.: 138777-25-0
Physical state: Solid
Color: White to yellow
Shipping: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage: Powder 4℃, protect from light, 2 years; In solvent -20℃ 1 month;

Solubility

soluble in DMSO

Documents

References

[1]. Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60(12):4638-41.

[2]. Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8.

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