BiochemProbe — Life Science Research Reagents

INT-777 free base · Synonyms: S-EMCA

INT-777 is a potent TGR5 agonist.

For research use only. Not for human or veterinary use.

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INT-777 structure

CAS No.: 1199796-29-6

Download structure (.mol)

Pack sizes & pricing

Size Price SKU Stock
25 mg USD 395.00 BP-01849-25mg In stock Get quote
50 mg USD 550.00 BP-01849-50mg In stock Get quote
100 mg USD 795.00 BP-01849-100mg In stock Get quote
250 mg USD 1,380.00 BP-01849-250mg In stock Get quote
500 mg USD 2,120.00 BP-01849-500mg In stock Get quote
1 g USD 3,280.00 BP-01849-1g In stock Get quote

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Description

INT-777 is a potent TGR5 agonist with an EC50 of 0.82 μM.

Biological activity

In Vitro INT-777 is a novel potent and selective TGR5 agonist with remarkable in vivo activity[1]. INT-777 (3 μM) increases ATP production in the human enteroendocrine cell line NCI-H716 in a cAMP-dependent manner. INT-777 (10 μM) lowers Isc and increases TEER when added on the serosal side of seromuscular stripped distal colon segments. INT-777 effect on basal secretion is reduced in neuron-free and TTX-treated mucosal-submucosal preparations. In Vivo INT-777 (1 μM/min/kg, p.o.) has a potent choleretic effect, prevents carboxyl CoA activation and subsequent conjugation, thereby favoring its chole-hepatic shunt pathway with a ductular absorption and a potent choleretic effect in HF-fed TGR5-Tg male mice. INT-777 (30 mg/kg/day, p.o.) increases energy expenditure and reduces hepatic steatosis and obesity upon high-fat feeding in TGR5-Tg mice.

Identifiers

SMILES
O[C@@H]1CC[C@@]2(C)[C@@]([C@@H](CC)[C@@H](O)[C@]3([H])[C@]2([H])C[C@H](O)[C@@]4(C)[C@@]3([H])CC[C@@H]4[C@H](C)C[C@H](C)C(O)=O)([H])C1
InChIKey
NPBCMXATLRCCLF-IRRLEISYSA-N

Specifications

MW (average)
450.6511
Formula
C27H46O5
CAS No.
1199796-29-6
Physical state
Solid
Color
white to yellow
Shipping
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage
Powder -20°C, 3 years , 4°C, 2 years ; In solvent -20°C, 1 month

Solubility

Soluble in DMSO : 100 mg/mL Ethanol : ≥ 50 mg/mL

References

[1]. Pellicciari R, et al. Discovery of 6alpha-ethyl-23(S)-methylcholic acid (S-EMCA, INT-777) as a potent and selective agonist for the TGR5 receptor, a novel target for diabesity. J Med Chem. 2009 Dec 24;52(24):7958-61.
[2]. Thomas C, et al. TGR5-mediated bile acid sensing controls glucose homeostasis. Cell Metab. 2009 Sep;10(3):167-77.
[3]. Duboc H, et al.Reduction of epithelial secretion in male rat distal colonic mucosa by bile acid receptor TGR5 agonist, INT-777: role of submucosal neurons. Neurogastroenterol Motil. 2016 Jun 3. doi: 10.1111/nmo.
[4]. Baiqiang Li, et al. INT-777, a bile acid receptor agonist, extenuates pancreatic acinar cells necrosis in a mouse model of acute pancreatitis. Biochem Biophys Res Commun. 2018 Sep 3;503(1):38-44.

Same research area

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