BiochemProbe — Life Science Research Reagents

P22077 free base · Synonyms: P-22077 · P 22077

P 22077 is a cell-permeable ubiquitin-specific protease 7 inhibitor.

For research use only. Not for human or veterinary use.

Target Deubiquitinase
Research area Cancer
Purity >98% Stock Inquire
Data Sheet SDS COA

P22077 structure

CAS No.: 1247819-59-5

Download structure (.mol)

Pack sizes & pricing

Size Price SKU Stock
100 mg USD 460.00 BP-02491-100mg In stock Get quote
250 mg USD 920.00 BP-02491-250mg In stock Get quote
500 mg USD 1,470.00 BP-02491-500mg In stock Get quote
1 g USD 2,360.00 BP-02491-1g In stock Get quote
2 g USD 3,770.00 BP-02491-2g In stock Get quote
3 g USD 4,520.00 BP-02491-3g In stock Get quote
5 g Inquire BP-02491-5g In stock Inquire

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Description

P 22077 is a cell-permeable ubiquitin-specific protease 7 (USP7) inhibitor with an EC50 of 8.01 μM. P 22077 also inhibits USP47 with an EC50 of 8.74 μM.

Biological activity

In Vitro P 22077 is an inhibitor of USP7 and DUB USP47, with EC50s of 8.01 μM and 8.74 μM, respectively. P 22077 (15-45 μM) inhibits a much smaller subset of DUBs. P 22077 (25 μM) causes DUBs inhibition in HEK293T cells[1]. P 22077 (0-20 μM) greatly reduces the cell viability of Neuroblastoma (NB) cells including IMR-32, NGP, CHLA-255, and SH-SY5Y cells but without NB-19 and SK-N-AS cells. P 22077 (10 μM) increases p53 activity and induces apoptosis in p53 wild-type and HDM2-expressing NB cells. P 22077 (5 μM) enhances the cytotoxic effect of Dox and VP-16 on NB cells, and enhances Dox- and VP-16-induced p53-mediated apoptosis. In Vivo P 22077 (15 mg/kg, i.p. 21 days) shows potent antitumor activities in an xenograft mouse model bearing IMR-32-derived tumors; P 22077 also exhibits antitumor effects after treatment at 10 mg/kg for 14 days in mice bearing SH-SY5Y-derived tumors, and at 20 mg/kg for 12 days in mice bearing NGP-derived tumors.

Identifiers

SMILES
CC(=O)C1=CC(=C(SC2=C(F)C=C(F)C=C2)S1)[N+]([O-])=O
InChIKey
RMAMGGNACJHXHO-UHFFFAOYSA-N

Specifications

MW (average)
315.3160
Formula
C12H7F2NO3S2
CAS No.
1247819-59-5
Physical state
Solid
Color
Light yellow to yellow
Shipping
Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage
Powder -20°C, 3 years , 4°C, 2 years ; In solvent -20°C, 1 year;

Solubility

Soluble in DMSO

Documents

References

[1].Altun M, et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem Biol. 2011 Nov 23;18(11):1401-12.
[2].Fan YH, et al. USP7 inhibitor P22077 inhibits neuroblastoma growth via inducing p53-mediated apoptosis. Cell Death Dis. 2013 Oct 17;4:e867.

Same target

Search Deubiquitinase

Same research area

Search Cancer

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